Journal of Rheumatic Diseases

Table. 2.

Primary antibodies used in IB, IF, and ChIP

Antigen Manufacturer Species Catalog number Dilution
Phos S536 NF-κB Cell signaling, Denvers, MA, USA Rabbit 3033 1:1,000 (IB)
NF-κB Cell signaling, Denvers, MA, USA Rabbit 8242 1:1,000 (IB)
1:50 (ChIP)
DKK1 Santa Cruz Biotechnology, Santa Cruz, TX, USA Mouse Sc-374574 1:100 (IF)
DKK1 Cell signaling, Denvers,MA, USA Rabbit 4687 1:1,000 (IB)
GAPDH Cell signaling, Denvers, MA, USA Rabbit 2118 1:1,000 (IB)

IB: immunoblotting, IF: immunostaining, ChIP: chromatin immunoprecipitation.

J Rheum Dis 2021;28:216~224 https://doi.org/10.4078/jrd.2021.28.4.216
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