Journal of Rheumatic Diseases

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Fig. 4. DKK1 overexpression promotes bone formation of enthesis. Enthesis cells were transfected with an empty vector or DKK1 plasmid and then stimulated to osteogenic differentiation. (A) Transfection efficiency was confirmed by RT-qPCR. As indicated days, differentiated cells were subjected to (B) ALP and COL staining, (C) ARS and VON staining, and (D) Hydroxyapatites staining. Representative images are shown. Scale bar is 200 μm. ALP: alkaline phosphatase, COL: collagen, ARS: Alizarin Red S, HA: hydroxyapatite, VON: Von Kossa.
J Rheum Dis 2021;28:216~224
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