Original

The Journal of the Korean Rheumatism Association 2003; 10(2): 141-150

Published online June 30, 2003

© Korean College of Rheumatology

류마티스 및 골관절염 섬유모세포양 활막세포에서 저산소증에 의한 Matrix Metalloproteinase 및 Tissue Inhibitor of Metalloproteinase-1 발현의 조절

차훈석·전찬홍·최화정*·안광성*·송영욱**·고은미

성균관대학교 의과대학 삼성서울병원 내과학교실, 삼성생명과학연구소*, 서울대학교 의과대학 내과학교실**

Regulation of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinase-1 Expression by Hypoxia in Rheumatoid and Osteoarthritis Fibroblast-Like Synoviocytes

Hoon-Suk Cha, M.D., Chan Hong Jeon, M.D., Hwa-Jung Choi*, Kwang-Sung Ahn, Ph.D.*, Yeong Wook Song, M.D.**, Eun-Mi Koh, M.D.

Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute*, Department of Internal Medicine, Seoul National University College of Medicine**, Seoul, Korea

Correspondence to : Eun-Mi Koh

Abstract

Objective: The rheumatoid synovium is a hypoxic environment and hypoxia has been implicated to have a role in the pathogenesis of rheumatoid arthritis (RA). In this work, we tried to investigate the effect of hypoxia on the expression of matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of metalloproteinase (TIMP)-1 in rheumatoid and osteoarthritis (OA) fibroblast-like synoviocytes (FLS).

Methods: FLS obtained from RA and OA patients were cultured under normoxic or hypoxic condition for 48 hours. Western blot analyses for MMP-1, -3 and TIMP-1 were performed. TIMP-1 mRNA levels were measured by Northern blot analysis.

Results: Hypoxia increased MMP-1 expression in rheumatoid FLS compared with normoxia.

TIMP-1 expression was decreased by hypoxia in rheumatoid FLS in both protein and mRNA levels. On the other hand, hypoxia did not significantly affect MMP-1, -3 and TIMP-1 expressions in OA FLS.

Conclusion: These results suggest that microenvironment such as hypoxia may directly contribute to joint destruction in RA by increasing the ratio of MMP-1 to TIMP-1 production in FLS.

Keywords Hypoxia, Rheumatoid arthritis, Matrix metalloproteinase, Tissue inhibitor of metalloproteinase

Article

Original

The Journal of the Korean Rheumatism Association 2003; 10(2): 141-150

Published online June 30, 2003

Copyright © Korean College of Rheumatology.

류마티스 및 골관절염 섬유모세포양 활막세포에서 저산소증에 의한 Matrix Metalloproteinase 및 Tissue Inhibitor of Metalloproteinase-1 발현의 조절

차훈석·전찬홍·최화정*·안광성*·송영욱**·고은미

성균관대학교 의과대학 삼성서울병원 내과학교실, 삼성생명과학연구소*, 서울대학교 의과대학 내과학교실**

Regulation of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinase-1 Expression by Hypoxia in Rheumatoid and Osteoarthritis Fibroblast-Like Synoviocytes

Hoon-Suk Cha, M.D., Chan Hong Jeon, M.D., Hwa-Jung Choi*, Kwang-Sung Ahn, Ph.D.*, Yeong Wook Song, M.D.**, Eun-Mi Koh, M.D.

Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute*, Department of Internal Medicine, Seoul National University College of Medicine**, Seoul, Korea

Correspondence to:Eun-Mi Koh

Abstract

Objective: The rheumatoid synovium is a hypoxic environment and hypoxia has been implicated to have a role in the pathogenesis of rheumatoid arthritis (RA). In this work, we tried to investigate the effect of hypoxia on the expression of matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of metalloproteinase (TIMP)-1 in rheumatoid and osteoarthritis (OA) fibroblast-like synoviocytes (FLS).

Methods: FLS obtained from RA and OA patients were cultured under normoxic or hypoxic condition for 48 hours. Western blot analyses for MMP-1, -3 and TIMP-1 were performed. TIMP-1 mRNA levels were measured by Northern blot analysis.

Results: Hypoxia increased MMP-1 expression in rheumatoid FLS compared with normoxia.

TIMP-1 expression was decreased by hypoxia in rheumatoid FLS in both protein and mRNA levels. On the other hand, hypoxia did not significantly affect MMP-1, -3 and TIMP-1 expressions in OA FLS.

Conclusion: These results suggest that microenvironment such as hypoxia may directly contribute to joint destruction in RA by increasing the ratio of MMP-1 to TIMP-1 production in FLS.

Keywords: Hypoxia, Rheumatoid arthritis, Matrix metalloproteinase, Tissue inhibitor of metalloproteinase

JRD
Jan 01, 2025 Vol.32 No.1, pp. 1~7
COVER PICTURE
Cumulative growth of rheumatology members and specialists (1980~2024). Cumulative distribution of the number of the (A) Korean College of Rheumatology members and (B) rheumatology specialists. (J Rheum Dis 2025;32:63-65)

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