The Journal of the Korean Rheumatism Association 2003; 10(2): 141-150
Published online June 30, 2003
© Korean College of Rheumatology
차훈석·전찬홍·최화정*·안광성*·송영욱**·고은미
성균관대학교 의과대학 삼성서울병원 내과학교실, 삼성생명과학연구소*, 서울대학교 의과대학 내과학교실**
Correspondence to : Eun-Mi Koh
Objective: The rheumatoid synovium is a hypoxic environment and hypoxia has been implicated to have a role in the pathogenesis of rheumatoid arthritis (RA). In this work, we tried to investigate the effect of hypoxia on the expression of matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of metalloproteinase (TIMP)-1 in rheumatoid and osteoarthritis (OA) fibroblast-like synoviocytes (FLS).
Methods: FLS obtained from RA and OA patients were cultured under normoxic or hypoxic condition for 48 hours. Western blot analyses for MMP-1, -3 and TIMP-1 were performed. TIMP-1 mRNA levels were measured by Northern blot analysis.
Results: Hypoxia increased MMP-1 expression in rheumatoid FLS compared with normoxia.
TIMP-1 expression was decreased by hypoxia in rheumatoid FLS in both protein and mRNA levels. On the other hand, hypoxia did not significantly affect MMP-1, -3 and TIMP-1 expressions in OA FLS.
Conclusion: These results suggest that microenvironment such as hypoxia may directly contribute to joint destruction in RA by increasing the ratio of MMP-1 to TIMP-1 production in FLS.
Keywords Hypoxia, Rheumatoid arthritis, Matrix metalloproteinase, Tissue inhibitor of metalloproteinase
The Journal of the Korean Rheumatism Association 2003; 10(2): 141-150
Published online June 30, 2003
Copyright © Korean College of Rheumatology.
차훈석·전찬홍·최화정*·안광성*·송영욱**·고은미
성균관대학교 의과대학 삼성서울병원 내과학교실, 삼성생명과학연구소*, 서울대학교 의과대학 내과학교실**
Hoon-Suk Cha, M.D., Chan Hong Jeon, M.D., Hwa-Jung Choi*, Kwang-Sung Ahn, Ph.D.*, Yeong Wook Song, M.D.**, Eun-Mi Koh, M.D.
Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute*, Department of Internal Medicine, Seoul National University College of Medicine**, Seoul, Korea
Correspondence to:Eun-Mi Koh
Objective: The rheumatoid synovium is a hypoxic environment and hypoxia has been implicated to have a role in the pathogenesis of rheumatoid arthritis (RA). In this work, we tried to investigate the effect of hypoxia on the expression of matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of metalloproteinase (TIMP)-1 in rheumatoid and osteoarthritis (OA) fibroblast-like synoviocytes (FLS).
Methods: FLS obtained from RA and OA patients were cultured under normoxic or hypoxic condition for 48 hours. Western blot analyses for MMP-1, -3 and TIMP-1 were performed. TIMP-1 mRNA levels were measured by Northern blot analysis.
Results: Hypoxia increased MMP-1 expression in rheumatoid FLS compared with normoxia.
TIMP-1 expression was decreased by hypoxia in rheumatoid FLS in both protein and mRNA levels. On the other hand, hypoxia did not significantly affect MMP-1, -3 and TIMP-1 expressions in OA FLS.
Conclusion: These results suggest that microenvironment such as hypoxia may directly contribute to joint destruction in RA by increasing the ratio of MMP-1 to TIMP-1 production in FLS.
Keywords: Hypoxia, Rheumatoid arthritis, Matrix metalloproteinase, Tissue inhibitor of metalloproteinase
Joong Kyung Ahn, M.D., Chan Hong Jeon, M.D., Jae Hyun Koh, M.D., Jin-Hee Kim*, Hwa-Jung Choi*, Kwang-Sung Ahn, Ph.D.*, Hoon-Suk Cha, M.D., Suk Hee Yu, M.D.**, Eun-Mi Koh, M.D.
The Journal of the Korean Rheumatism Association 2003; 10(1): 30-38Byeongzu Ghang, M.D., Ph.D., Jin Kyun Park, M.D., Ph.D., Ji Hyeon Ju, M.D., Ph.D., Seungwoo Han, M.D., Ph.D.
J Rheum Dis -0001; ():Shohei Anno, M.D., Kentaro Inui, M.D., Ph.D., Masahiro Tada, M.D., Ph.D., Yuko Sugioka, M.D., Ph.D., Tadashi Okano, M.D., Ph.D.,, Kenji Mamoto, M.D., Ph.D., Tatsuya Koike, M.D., Ph.D.
J Rheum Dis -0001; ():